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Zeiss LSM 980 Inverted Spectral Confocal

Technical description  

Microscope Stand: Zeiss Axio Observer 

Available Objectives:

Magnification Medium Numerical Aperture Working Distance (mm) Correction Collar
2.5x Air 0.085 N/A N
10x Air 0.45 4.0 N
20x Air 0.80 0.55 N
63x Oil 1.40 0.19 N

Illumination modes: Transmitted brightfield, widefield fluorescence, confocal fluorescence

Camera: Hamamatsu Orca Flash 4.0

Widefield Filters:

  • DAPI (ex: 359/24, BS 395, em: LP 420)
  • eGFP (ex:  470/20, BS 495, em: 525/25)
  • HE mPlum (ex: 587/13, BS 605, em: 647/35)

Laser Lines:  405 nm, 445, 488 nm, 514 nm, 561 nm, 594 nm, 639 nm and 730 nm.

Confocal Detectors:

  • 1x Multialkali PMT with tunable detection (378 – 667 nm)
  • 1x Multialkali PMT with tunable detection (437 – 757 nm)
  • 32x GaAsP PMT spectral array (446 – 694 nm)
  • 2 x Near IR PMTs (660 – 705 nm & 755 – 900 nm)
  • Airyscan super-resolution detector (DAPI, FITC, TRITC, Cy5)

Other features

  • AI sample finder
  • Definite focus
  • Tokai Hit live-cell incubation chamber