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Zeiss Celldiscoverer 7

The Celldiscoverer is an advanced automated imaging platform, ideal for multi-well plates, slides and live-cell imaging. This inverted microscope is a closed-box solution that automates image acquisition and analysis at every step. Through motorized correction collars, a sample finder and thickness detector, the instrument automatically compensates for different sample holders, sample thicknesses and immersion media, allowing for superior imaging quality in plastic well plates (opposed to glass), tissue and cell scaffolds.

Technical description  

Available Objectives:

Magnification Medium Numerical Aperture Working Distance (mm)  

Motorized Correction Collar

2.5, 5x or 10x Air 0.12, 0.25 or 0.35 N
10x, 20x or 40x Air 0.35 or 0.70 Y
25x, 50x or 100x Water 1.2 Y

Illumination modes: Fluorescence and brightfield

Excitation wavelengths: Colibri light sources with the following LEDs:

  • 385/30 (DAPI / Hoechst)
  • 423/44 (CFP / AF430)
  • 469/38 (GFP / AF488)
  • 511/44 (eYFP / AF514)
  • 555/30 (TdTomato / AF555)
  • 591/27 (mCherry / AF594)
  • 631/33 (PSmOrange / AF647)

Filters:

  • Quad Bandpass Filter Set
    • Excitation: 405 + 493 + 575 + 653 nm
    • Emission: 425/30 + 521/30 + 592/25 + 709/100 nm
  • Triple Bandpass Filter Set
    • Excitation: 450 + 538 + 510
    • Emission: 467/24 + 555/25 + 687/145

Cameras: Axiocam 712 monochrome

Other features:

  • Automatic objective immersion
  • Sample finder, providing image of sample and holder and automatically focusing
  • Motorized correction collars, enhancing imaging in plastic well-plates and thick samples
  • ZenConnect, contextualizing acquired images with the whole sample, allowing for correlative work
  • Live cell incubation (CO2, humidity, and temperature control)
  • Image deconvolution