Technical description
Microscope Stand: Imager Z2
Available Objectives:
Magnification | Medium | Numerical Aperture | Working Distance (mm) | Correction Collar |
5x | Air | 0.13 | 15.8 | N |
10x | Air | 0.45 | 2.1 | N |
20x | Air | 0.80 | 0.55 | N |
50x | Air | 0.55 | 9.1 | N |
63x | Oil | 1.40 | 0.19 | N |
100x | Air | 0.75 | 4.0 | N |
Illumination modes: Transmitted brightfield, widefield fluorescence, confocal fluorescence
Camera: Axiocam 712 mono
Widefield Filters:
- DAPI (ex: 359/25, em: 445/25)
- eGFP (ex: 470/20, em: 525/25)
- DsRed (ex: 545/13, em: 605/35)
Laser lines: 405, 445, 488, 561, 639 and 730 nm
Confocal Detectors:
- 3 x PMTs with tunable detection (379 – 736 nm)
- 2 x Near IR PMTs (642 – 730 nm & 730 – 900 nm)
- Airyscan super-resolution detector (DAPI, FITC, TRITC, Cy5, Cy7)
Other features
- Linkam cryo stage for TEM grids
- Emission spectral scanning (379 – 736 nm)
- LSM+ image deconvolution
- Photon counting