Technical description
Microscope Stand: Zeiss Axio Observer
Available Objectives:
Magnification | Medium | Numerical Aperture | Working Distance (mm) | Correction Collar |
2.5x | Air | 0.085 | N/A | N |
10x | Air | 0.45 | 4.0 | N |
20x | Air | 0.80 | 0.55 | N |
63x | Oil | 1.40 | 0.19 | N |
Illumination modes: Transmitted brightfield, widefield fluorescence, confocal fluorescence
Camera: Hamamatsu Orca Flash 4.0
Widefield Filters:
- DAPI (ex: 359/24, BS 395, em: LP 420)
- eGFP (ex: 470/20, BS 495, em: 525/25)
- HE mPlum (ex: 587/13, BS 605, em: 647/35)
Laser Lines: 405 nm, 445, 488 nm, 514 nm, 561 nm, 594 nm, 639 nm and 730 nm.
Confocal Detectors:
- 1x Multialkali PMT with tunable detection (378 – 667 nm)
- 1x Multialkali PMT with tunable detection (437 – 757 nm)
- 32x GaAsP PMT spectral array (446 – 694 nm)
- 2 x Near IR PMTs (660 – 705 nm & 755 – 900 nm)
- Airyscan super-resolution detector (DAPI, FITC, TRITC, Cy5)
Other features
- AI sample finder
- Definite focus
- Tokai Hit live-cell incubation chamber