Technical description
Available Objectives:
| Magnification | Medium | Numerical Aperture | Working Distance (mm) |
Motorized Correction Collar |
| 2.5, 5x or 10x | Air | 0.12, 0.25, 0.35 | – | N |
| 10x, 20x or 40x | Air | 0.35, 0.70, 0.70 | – | Y |
| 25x, 50x or 100x | Water | 1.2 | – | Y |
Illumination modes: Fluorescence and brightfield
Excitation wavelengths: Colibri light sources with the following LEDs:
- 385/30 (DAPI / Hoechst)
- 423/44 (CFP / AF430)
- 469/38 (GFP / AF488)
- 511/44 (eYFP / AF514)
- 555/30 (TdTomato / AF555)
- 591/27 (mCherry / AF594)
- 631/33 (PSmOrange / AF647)
Filters:
- Quad Bandpass Filter Set
- Beamsplitter: 405 + 493 + 575 + 653 nm
- Emission: 425/30 + 521/30 + 592/25 + 709/100 nm
- Triple Bandpass Filter Set
- Beamsplitter: 450 + 538 + 510
- Emission: 467/24 + 555/25 + 687/145
Cameras: Axiocam 712 monochrome
Other features:
- Automatic objective immersion
- Sample finder, providing image of sample and holder and automatically focusing
- Motorized correction collars, enhancing imaging in plastic well-plates and thick samples
- ZenConnect, contextualizing acquired images with the whole sample, allowing for correlative work
- Live cell incubation (CO2, humidity, and temperature control)
- Image deconvolution