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Inverted Confocal (Zeiss LSM 700)

Technical description  

Microscope Stand: Zeiss Axio Observer 

Available Objectives:

Magnification Medium Numerical Aperture Working Distance (mm) Correction Collar
10x Air 0.30 4.0 N
20x Air 0.80 0.55 N
40x Oil 1.40 0.13 N
63x Oil 1.40 0.19 N

Laser Lines:  405 nm, 488 nm, 555 nm, 639 nm 

Filters: Continuous wavelength splitter between two PMTs that possess either SP (490, 555, 640) or LP (490, 560, 640) filters for DAPI, FITC, TRITC and Cy5 channels

Detectors: 2 high sensitivity PMTs 

Camera: Zeiss HrM